the staphy lococcal exfoliative toxin development of a modified assay system
نویسندگان
چکیده
certain strains of staphylococcus aureus produce a toxin which cause epidermal exfoliation within 18 hours after direct subcutaneous or intraperitoneal injection into newborn mice. the extracellular product responsible for exfoliation is termed exfoliative toxin (et). in the present studies a modified assay system has been developed for detection of exfoliativeactivity in toxin negative strains as determined by standa rd method of melish and glasgow. if extracellular supernatant material from naturally occuring et negative st rains was concentrated 20 fold and inoculated into newborn mice, exfoliative toxin act ivity could be detected,
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Exfoliative toxin (ET) from a phage group II Staphylococcus aureus strain causing staphylococcal scalded-skin syndrome was purified by electrofocusing. Ampholytes and salts were removed from the final product by column chromatography on G-50 Sephadex. Sodium dodecyl sulfate-polyacrylamide gels of the final product yielded a single band upon gel electrophoresis, even when 60 mug of protein was p...
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عنوان ژورنال:
acta medica iranicaجلد ۱۹۷۶، شماره ۱۲، صفحات ۴۷-۵۲
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